Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS
نویسندگان
چکیده
منابع مشابه
Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway.
RpoS and RpoN are two alternative sigma factors typically associated with general stress responses in bacteria. To date, there has been no experimental evidence that RpoS and RpoN can directly control the expression of one another. Herein, using a combined strategy of gene disruption and genetic complementation targeting rpoN and rpoS in Borrelia burgdorferi strain 297, we describe a regulatory...
متن کاملEvidence that RpoS ( S ) in Borrelia burgdorferi Is Controlled Directly by RpoN ( 54 / N )
Lyme disease, caused by Borrelia burgdorferi, is the most commonly reported arthropod-borne disease in both the United States and Europe (32). At the molecular level, certain membrane lipoproteins of B. burgdorferi are vital for maintaining the spirochete in its zoonotic transmission cycle between ticks and mammals. For example, the reciprocal regulation of outer surface (lipo)proteins A (OspA)...
متن کاملAltered stationary-phase response in a Borrelia burgdorferi rpoS mutant.
The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrB(r) as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase...
متن کاملInsights into the complex regulation of rpoS in Borrelia burgdorferi
Co-ordinated regulation of gene expression is required for the transmission and survival of Borrelia burgdorferi in different hosts. The sigma factor RpoS (sigma(S)), as regulated by RpoN (sigma(54)), has been shown to regulate key virulence factors (e.g. OspC) required for these processes. As important, multiple signals (e.g. temperature, pH, cell density, oxygen) have been shown to increase t...
متن کاملIncreasing RpoS Expression Causes Cell Death in Borrelia burgdorferi
RpoS, one of the two alternative σ factors in Borrelia burgdorferi, is tightly controlled by multiple regulators and, in turn, determines expression of many critical virulence factors. Here we show that increasing RpoS expression causes cell death. The immediate effect of increasing RpoS expression was to promote bacterial division and as a consequence result in a rapid increase in cell number ...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2009
ISSN: 1471-2180
DOI: 10.1186/1471-2180-9-108